Evaluation of specific RBE in different cells of hippocampus under high-dose proton irradiation in rats.

The study aimed to determine the specific relative biological effectiveness (RBE) of various cells in the hippocampus following proton irradiation. Sixty Sprague-Dawley rats were randomly allocated to 5 groups receiving 20 or 30 Gy of proton or photon irradiation. Pathomorphological neuronal damage in the hippocampus was assessed using Hematoxylin-eosin (HE) staining. The expression level of NeuN, Nestin, Caspase-3, Olig2, CD68 and CD45 were determined by immunohistochemistry (IHC). The RBE range established by comparing the effects of proton and photon irradiation at equivalent biological outcomes. Proton20Gy induced more severe damage to neurons than photon20Gy, but showed no difference compared to photon30Gy. The RBE of neuron was determined to be 1.65. Similarly, both proton20Gy and proton30Gy resulted in more inhibition of oligodendrocytes and activation of microglia in the hippocampal regions than photon20Gy and photon30Gy. However, the expression of Olig2 was higher and CD68 was lower in the proton20Gy group than in the photon30Gy group. The RBE of oligodendrocyte and microglia was estimated to be between 1.1 to 1.65. For neural stem cells (NSCs) and immune cells, there were no significant difference in the expression of Nestin and CD45 between proton and photon irradiation (both 20 and 30 Gy). Therefore, the RBE for NSCs and immune cell was determined to be 1.1. These findings highlight the varying RBE values of different cells in the hippocampus in vivo. Moreover, the actual RBE of the hippocampus may be higher than 1.1, suggesting that using as RBE value of 1.1 in clinical practice may underestimate the toxicities induced by proton radiation.

Altered hydrophobicity and membrane composition in stress-adapted Listeria innocua.

Exposure of Listeria innocua to acid and starvation stress decreases sensitivity to the quaternary ammonium compound cetrimide, whereas exposure to cold and heat stress increases sensitivity to this compound. Changes in membrane lipids occur in response to certain types of stress, and these changes likely impact cell sensitivity to chemical sanitizers. The present study included an assessment of the effects of acid, starvation, cold, and heat stress on net cell hydrophobicity and fatty acid composition in L. innocua. Net cell hydrophobicity was determined by measuring absorbance of stress-adapted cell suspensions after partitioning with the nonpolar solvent n-hexadecane. Free fatty acids extracted from stress-adapted suspensions were analyzed by gas chromatography. Adaptation to acid and starvation increased net cell hydrophobicity and decreased membrane fluidity, which was correlated with reductions in anteiso fatty acids and in ratios of anteiso to iso fatty acids. Conversely, cold-stressed populations exhibited decreased net cell hydrophobicity and increased membrane fluidity with a corresponding increase in C15:C17 and anteiso:iso ratios and in C18 unsaturated fatty acids. No significant changes in net cell hydrophobicity or membrane fluidity were observed in heat-stressed cells, which exhibited increased sensitivity to cetrimide, suggesting another mechanism for altered cell sensitivity. These findings indicate that the efficacy of cetrimide against Listeria is partially dependent on the physiological state of the organism following exposure to various environmental stresses.